The Irish Community Rapid Response (ICRR) Air Ambulance, based in Mallow, could be grounded within days without immediate support.
The service, in operation less than six months, needs the immediate donation of €400,000 to keep its services flying across the country.
“The ICRR Air Ambulance has flown on mission over 250 times in just five months of service,” ICRR Operations Manager Ruth Bruton said.
“The success of the Air Ambulance has meant we’ve flown an additional 17 missions per month on average, which means increased costs to keep us flying.
“We’ve already helped in excess of 200 patients, their families and their communities by responding rapidly to them when they need help the most. These missions have been in both challenging rural locations and in towns and cities right across the country.
"We estimate over 600 missions could be flown in 2020 and those 600 lifesaving missions are in jeopardy without immediate funding.”
The ICRR Air Ambulance needs funds for the provision of the helicopters, pilots, air base facilities, insurance, firefighting and other operational needs to provide the vital service.
“The ICRR Air Ambulance could be called on to help you or your loved one next,” Ms Bruton added.
“I’m asking on behalf of the people we can save in the future, please ensure that we are there to help.